Developing assisted reproductive technologies for Australian amphibians

Amphibians are experiencing unprecedented declines worldwide, with recent estimates putting ~40% of amphibians at risk of extinction. Within Australia, 46 species (approximately 20% of all Australian frogs) are either extinct or threatened with extinction.

This project aims to develop protocols for assisted reproductive technologies (ARTs), such as the long term storage of sperm by cryopreservation, IVF and hormonal induction of sperm. The project has emphasis on the endangered green and golden bell frog (Litoria aurea) but aims to aid all amphibians. It is hoped that development of these protocols will contribute to genetic rescue of endangered species with the use of biobanking of sperm.

For more information and published research visit:
https://www.researchgate.net/project/Developing-assisted-reproductive-technologies-for-Australian-amphibians

An endangered green and golden bell frog (Litoria aurea) produced from cryopreserved sperm. Photo: Cassandra Bugir

Publications

Differential success in obtaining gametes between male and female Australian temperate frogs by hormonal induction: A review.

John Clulow, Melissa Pomering, Danielle Herbert, Rose Upton, Natalie E. Calatayud, Simon Clulow, Michael J. Mahony, Vance L. Trudeau

General and Comparative Endocrinology, vol. 265, 2018 Jul 31, pp. 141-148

Sperm collection and storage for the sustainable management of amphibian biodiversity.

Robert K. Browne, Aimee J. Silla, Rose Upton, Gina Della-Togna, Ruth Marcec-Greaves, Natalia V. Shishova, Victor K. Uteshev, Belin Proaño, Oscar D. Pérez, Nabil Mansour, Svetlana A. Kaurova, Edith N. Gakhova, Jacky Cosson, Borys Dyzuba, Ludmila I. Kramarova, Dale McGinnity, Manuel Gonzalez, John Clulow, Simon Clulow

Theriogenology, vol. 133, 2019 Jun 14, pp. 187-200

Integrating biobanking could produce significant cost benefits and minimise inbreeding for Australian amphibian captive breeding programs

Lachlan G. Howell, Peter R. Mawson, Richard Frankham, John C. Rodger, Rose M. O. Upton, Ryan R. Witt, Natalie E. Calatayud, Simon Clulow, John Clulow

Reproduction, Fertility and Development, vol. 33, 2021 Apr 17, pp. 573–587

Generation of a sexually mature individual of the Eastern dwarf tree frog, Litoria fallax, from cryopreserved testicular macerates: proof of capacity of cryopreserved sperm derived offspring to complete development.

Rose Upton, Simon Clulow, Michael J. Mahony, John Clulow

Conservation Physiology, vol. 6(1), 2017 Nov 31

Amphibian Assisted Reproductive Technologies: Moving from Technology to Application.

John Clulow, Rose Upton, Vance L. Trudeau, Simon Clulow

Advances in Experimental Medicine and Biology, vol. 1200, 2018 Nov 31, pp. 413-463

Generation of reproductively mature offspring from the endangered green and golden bell frog Litoria aurea using cryopreserved spermatozoa.

Rose Upton, Simon Clulow, Natalie E. Calatayud, Kim Colyvas, Rebecca G. Y. Seeto, Lesley A. M. Wong, Michael J. Mahony, John Clulow

Reproduction, Fertility and Development, vol. 33, 2021 Mar 5, pp. 562–572

Rose Upton

Developing assisted reproductive technologies for Australian amphibians

Publications

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